Studies of budding and cell wall structure of yeast; electron microscopy of the sections.

Observations of intact yeast cells with the electron microscope have yielded little information on cellular structure because of the impenetrability of the cells to the electron beam. The development of techniques for the preparation of ultra-thin sections promises to obviate this difficulty, and several papers have already appeared in which this approach has been applied in studies of the structure of microorganisms (Chapman and Hillier, 1953; Birch-Anderson et al., 1953; Wolken and Palade, 1953; Gustafson et al., 1954). In this paper we describe details of the budding process and the structure of the cell wall of Saccharomyces cerevisiae as observed in thin sections of intact cells and purified cell walls.